effect-artificial-tear-formulations-on-metabolic-activity-human

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Artificial lipid-containing tear formulations are developed to reduce tear evaporation by the restoration of a deficient tear lipid layer. Artificial tear ...

University of Waterloo

: This study investigated kinetic lipid uptake to four silicone hydrogel (SiHy) lenses over a period of four weeks, using an  radiolabel method.: Four contemporary monthly replacement SiHy lenses (lotrafilcon B, senofilcon C, comfilcon A, samfilcon A) were incubated in three different solutions: 1) An artificial tear solution (ATS) containing C-labeled phosphatidylcholine (PC), 2) an ATS containing C-cholesteryl oleate (CO) and 3) an ATS containing four C-radiolabeled lipids (PC, phosphatidylethanolamine, CO, and cholesterol (total lipid)). After 16 hours, lipids were extracted twice from the lenses with chloroform:methanol and the radioactive counts determined the lipid quantities to simulate 1 day of wear. OPTI-FREE PureMoist (Alcon) was used to clean and disinfect the remaining lenses daily and the lipid quantities were further determined after 2 weeks and 4 weeks.: The amount of total lipid increased for all lenses over time ( < .01). After four weeks, total lipid accumulated was 20.26 ± 0.15 µg/lens for senofilcon C, which was significantly higher ( < .01) than all other lens materials (samfilcon A - 17.84 ± 0.21; comfilcon A - 16.65 ± 0.12; lotrafilcon B - 7.41 ± 0.56 µg/lens). CO was highest on lotrafilcon B (1.26 ± 0.13 µg/lens) and senofilcon C attracted the most PC (3.95 ± 0.12 µg/lens) compared to the other materials.: The amount of both polar and non-polar lipid deposition on monthly replacement SiHy lenses increased over 4 weeks, with significant differences being seen between lens materials.

Adeline Suko

Centre for Ocular Research and Education (CORE),
School of Optometry & Vision Science,
Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science

University of Waterloo

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Adeline Suko

Centre for Ocular Research and Education (CORE),School of Optometry & Vision Science,Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science

University of Waterloo

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation

Centre for Ocular Research and Education (CORE),
School of Optometry & Vision Science,
Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science