immunomagnetic-isolation-vascular-wall-resident-cd34-stem-cells-from

Immunomagnetic Isolation of the Vascular Wall-Resident CD34<sup>+</sup> Stem Cells from Mice

Resident CD34+ vascular wall-resident stem and progenitor cells (VW-SCs) are increasingly recognized for their crucial role in regulating vascular injury ...

development-of-a-murine-model-for-femoral-artery-anastomotic-stenosis

Development of a Murine Model for Femoral Artery Anastomotic Stenosis

In vascular surgery, vascular anastomosis is a common reconstructive technique used to restore blood flow. However, anastomotic restenosis is a frequent ...

southwest medical university

Nearly all physiological processes are controlled at some level by G-protein-coupled receptor (GPCR) signaling activity. The thromboxane A2 (TXA2) receptor (TP) is a member of the GPCR family. The ultimate effect of TP receptor activation depends on the availability of specific G proteins, which in turn depend on the cell type, tissue, and disease state. However, the roles of the TXA2-TP signaling pathway executed under disease states are poorly defined. In this study, 16-week-spontaneously hypertensive rats (SHR), the 18-month-SHR (OldSHR), and the age-matched Wistar-Kyoto (WKY) rats were used to study the vasoconstriction of mesenteric resistance artery induced by TP-specific agonist, U-46619. Vasoconstriction induced by U-46619 was significantly attenuated in OldWKY and OldSHR rats, and mesenteric arteries with impaired response to U-46619 responded strongly to the adrenergic receptor agonist, phenylephrine. Similar vascular responses to U-46619 were obtained in endothelium-denuded mesenteric arteries. Accordingly, the expression of TP membrane proteins in mesenteric vessels was decreased, and the endogenous TP competitor, 8, 9-EET, in serum was increased, which was partly responsible for the decreased vascular reactivity of U-46619. Decreased TP membrane expression was associated with TP endocytosis, which involved actin cytoskeletal remodeling, including increased ratio of F-actin/G-actin in OldWKY and OldSHR rats. Hence, we studied the effects of TXA2 and its receptors on blood vessels and found that the TXA2-TP prostaglandin signaling pathway was impaired in older adults, which would facilitate the creation of "precision therapeutics" that possess selective efficacy in diseases.

Thromboxane-induced contractile response of mesenteric arterioles is diminished in the older rats and the older hypertensive rats.

Vascular resident stem cells expressing stem cell antigen-1 (Sca-1 cells) promote vascular regeneration and remodelling following injury through migration, proliferation and differentiation. The aim of this study was to examine the contributions of ATP signalling through purinergic receptor type 2 (P2R) isoforms in promoting Sca-1 cell migration and proliferation after vascular injury and to elucidate the main downstream signalling pathways.

ATP purinergic receptor signalling promotes Sca-1 cell proliferation and migration for vascular remodelling.

Inward rectifier potassium channels (Kir channels) exist in a variety of cells and are involved in maintaining resting membrane potential and signal transduction in most cells, as well as connecting metabolism and membrane excitability of body cells. It is closely related to normal physiological functions of body and the occurrence and development of some diseases. Although the functional expression of Kir channels and their role in disease have been studied, they have not been fully elucidated. In this paper, the functional expression of Kir channels in vascular endothelial cells and smooth muscle cells and their changes in disease states were reviewed, especially the recent research progress of Kir channels in stem cells was introduced, in order to have a deeper understanding of Kir channels in vascular tissues and provide new ideas and directions for the treatment of related ion channel diseases.

Chunshu Li

southwest medical university

Immunomagnetic Isolation of the Vascular Wall-Resident CD34⁺ Stem Cells from Mice

Development of a Murine Model for Femoral Artery Anastomotic Stenosis

Chunshu Li

southwest medical university

Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice

Development of a Murine Model for Femoral Artery Anastomotic Stenosis

Immunomagnetic Isolation of the Vascular Wall-Resident CD34⁺ Stem Cells from Mice