נתמך על ידי NIH HL081171, NIH HL080203

<ol>
	<li>Bolar, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+regional+pulmonary+blood+flow+using+ASL-FAIRER.">Quantification of regional pulmonary blood flow using ASL-FAIRER.</a> Magn Reson Med. 55, 1308-1317 (2006).</li><li>Henderson, A. C., Prisk, G. K., Levin, D. L., Hopkins, S. R., Buxton, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterizing+pulmonary+blood+flow+distribution+measured+using+arterial+spin+labeling.">Characterizing pulmonary blood flow distribution measured using arterial spin labeling.</a> NMR Biomed. 22, 1025-1035 (2009).</li><li>Theilmann, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+MRI+measurement+of+lung+density+must+account+for+the+change+in+T(2)+(*)+with+lung+inflation.">Quantitative MRI measurement of lung density must account for the change in T(2) (*) with lung inflation.</a> J Magn Reson Imaging. 30, 527-534 (2009).</li><li>Hopkins, S. R., Garg, J., Bolar, D. S., Balouch, J., Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+blood+flow+heterogeneity+during+hypoxia+and+high-altitude+pulmonary+edema.">Pulmonary blood flow heterogeneity during hypoxia and high-altitude pulmonary edema.</a> Am J Respir Crit Care Med. 171, 83-87 (2005).</li><li>Miller, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Standardisation+of+spirometry.">Standardisation of spirometry.</a> Eur Respir J. 26, 319-338 (2005).</li><li>Hopkins, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vertical+gradients+in+regional+lung+density+and+perfusion+in+the+supine+human+lung:+the+Slinky+effect.">Vertical gradients in regional lung density and perfusion in the supine human lung: the Slinky effect.</a> J Appl Physiol. 103, 240-248 (2007).</li><li>Arai, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypoxic+pulmonary+vasoconstriction+does+not+contribute+to+pulmonary+blood+flow+heterogeneity+in+normoxia+in+normal+supine+humans.">Hypoxic pulmonary vasoconstriction does not contribute to pulmonary blood flow heterogeneity in normoxia in normal supine humans.</a> J Appl Physiol. 106, 1057-1064 (2009).</li><li>Dawson, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+pulmonary+vasomotion+in+physiology+of+the+lung.">Role of pulmonary vasomotion in physiology of the lung.</a> Physiol Rev. 64, 544-616 (1984).</li><li>Prisk, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+perfusion+in+the+prone+and+supine+postures+in+the+normal+human+lung.">Pulmonary perfusion in the prone and supine postures in the normal human lung.</a> J Appl Physiol. 103, 883-894 (2007).</li><li>Henderson, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steep+head-down+tilt+has+persisting+effects+on+the+distribution+of+pulmonary+blood+flow.">Steep head-down tilt has persisting effects on the distribution of pulmonary blood flow.</a> J Appl Physiol. 101, 583-589 (2006).</li><li>Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+age+on+pulmonary+perfusion+heterogeneity+measured+by+magnetic+resonance+imaging.">Effects of age on pulmonary perfusion heterogeneity measured by magnetic resonance imaging.</a> J Appl Physiol. 102, 2064-2070 (2007).</li><li>Wasserman, K. H., Sue, D., Casaburi, R., Whipp, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calculations,+Fomulae,+and+Examples+(Appendix+C).">Calculations, Fomulae, and Examples (Appendix C).</a> Principles of Exercise Testing and Interpretation. , (1999).</li></ol>

אין ניגודי אינטרסים הכריז.

שיטה זו מאפשרת מדידה של השפעת ריכוז החמצן השראה על התפלגות מרחבית של זרימת דם ריאתי באמצעות טכניקות פיזיולוגיים בסיסיים בסביבת MR סריקה. שימוש בטכניקות פיזיולוגיות בשילוב עם הדמיה פרוטון כמותי של הריאות הוא מיושם בקלות יחסית. כד?...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><th> ציוד </th><th> חברה </th><th> מודל </th></tr><tr><td> MRI </td><td> GE </td><td> 1.5 T-GE HDX EXICITE twinspeed סורק </td></tr><tr><td> מטבולית בעגלה </td><td> ParvoMedics </td><td> TrueOne 2400 </td></tr><tr><td> Pulse Oximeter </td><td> פולשנית </td><td> 7500 ל </td></tr><tr><td> מכשיר המדידה פועל </td><td> טכנולוגיות רפואיות אנדובר </td><td> EasyOne מכשיר המדידה פועל diagonostic </td></tr><tr><td> מסכה </td><td> הנס רודולף &amp; </td><td> 7400 סדרה Oro-באף מסכה, קטן בינוני, ו גדול </td></tr><tr><td> שסתום </td><td> הנס רודולף &amp; </td><td> דו כיוונית ללא rebreathing שסתומים T-Shape ™ תצורה, 2600 בינונית. גדול 2700 </td></tr><tr><td> ראש הגדר </td><td> הנס רודולף &amp; </td><td> כובע ראש (גודל למבוגרים), רצועת הקליפים &amp; נעילה. </td></tr><tr><td> פניאומטיים כיווני שסתום בקרה בקר </td><td> הנס רודולף &amp; </td><td> בוכנה בודדת הזזה-Type ™ השסתום בקר 4285A </td></tr><tr><td> Non-לשדר אוסף גז שקית </td><td> הנס רודולף &amp; </td><td> 6100 (100 ליטר). </td></tr><tr><td> שפופרת </td><td> VacuMed </td><td> ניקוי בור Tubing 108 &quot;, 1-3/8&quot; OD אביזרי </td></tr><tr><td> פאנטום </td><td> מנטור </td><td> ברסט שתל עגול, 250cc </td></tr><tr><td> matlab </td><td> MathWorks </td><td></td></tr></tbody></table>

magnetic resonance imaging quantification of pulmonary perfusion using calibrated arterial spin labeling

זה מדגים שיטה הדמיה MR למדוד את הפריסה המרחבית של זרימת דם ריאתי בנבדקים בריאים במהלך normoxia (בהשראת O 2, חלק (F אני O 2) = 0.21), היפוקסיה (F אני O 2 = 0.125), ו hyperoxia (F אני O 2 = 1.00). בנוסף, התגובות הפיזיולוגיות של הנבדק מנוטרים בסביבה MR סריקה. תמונות MR התקבלו על 1.5 T סורק MRI במהלך GE להחזיק נשימה של פרוסת sagittal בריאה הנכון יכולת שיורית תפקודית. ספין עורקי תיוג רצף (ASL-הוגן) שימש כדי למדוד את הפריסה המרחבית של 1,2 זרימת דם ריאתי רב הד מהירה שיפוע הד (mGRE) רצף 3 שימש לכמת את פרוטון אזורית (כלומר H 2 O) צפיפות, המאפשר כימות זלוף צפיפות מנורמל עבור כל voxel (מיליליטר דם לדקה לכל רקמת הריאה גרם). עם פנאומטי החלפת השסתום facemask מצוידים שסתום שאינו rebreathing 2-way, ריכוזי חמצן שונים הוכנסו הנושא הסורק MR דרך צינורות הגז השראה. עגלת מטבולית אסף גז דרך צינורות נשיפה נשיפה. מעורב הנשיפה O 2 ו-CO 2 בריכוז, צריכת חמצן, פחמן דו חמצני הייצור, יחס החליפין הנשימה, תדירות הנשימה ואת נפח גאות נמדדו. קצב הלב רוויון החמצן נוטרו באמצעות הדופק oximetry. נתונים שהתקבלו בנושא נורמלי הראו כי, כצפוי, קצב הלב היה גבוה יותר היפוקסיה (60 BPM) מאשר במהלך normoxia (51) או hyperoxia (50) ואת הרוויה חמצן עורקי (SpO 2) הופחת במהלך היפוקסיה ל -86%. ממוצע אוורור היה 8.31 L / min BTPS במהלך היפוקסיה, 7.04 L / min במהלך normoxia, ו 6.64 L / min במהלך hyperoxia. נפח גאות ושפל היה 0.76 במהלך L L, 0.69 היפוקסיה במהלך normoxia, ו 0.67 ליטר במהלך hyperoxia. נציג לכמת נתונים ASL הראו כי צפיפות זלוף אומר מנורמל היה 8.86 מ&quot;ל / דקה / g במהלך היפוקסיה, 8.26 מ&quot;ל / דקה / g במהלך normoxia ו 8.46 מ&quot;ל / דקה / g במהלך hyperoxia, בהתאמה. בנושא זה, הפיזור היחסי 4, מדד ההטרוגניות העולמי, עלה ב היפוקסיה (חוסר חמצן במהלך 1.07, 0.85 במהלך normoxia, ו 0.87 במהלך hyperoxia) ואילו ממד פרקטלית (DS), אחר מדד ההטרוגניות המשקפת מבנה הסתעפות כלי הדם, נותר ללא שינוי (1.24 במהלך היפוקסיה, במהלך normoxia 1.26, 1.26 ו במהלך hyperoxia). סקירה כללית. פרוטוקול זה יהיה להדגים את רכישת נתונים כדי למדוד את חלוקת זלוף ריאתי noninvasively בתנאים של normoxia, היפוקסיה, ו hyperoxia באמצעות תהודה מגנטית טכניקה המכונה תיוג ספין עורקים (ASL). רציונל: מדידת זרימת דם ריאתי פרוטון צפיפות ריאות באמצעות טכניקה MR מציעה תמונות ברזולוציה מרחבית גבוהה אשר ניתן לכמת את היכולת לבצע מדידות חוזרות תחת מספר תנאים פיזיולוגיים שונים. במחקרים האדם, PET, SPECT, ו-CT משמשות גם טכניקות חלופיות. עם זאת, טכניקות אלה כרוכות בחשיפה לקרינה מייננת, ולכן אינם מתאימים מדידות חוזרות בבני אדם.

Watch this Scientific Journal Video about Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling at JoVE.com

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

שיטת הדמיה MR ללמוד את התפלגות זרימת דם ריאתי תחת מגוון רחב של מצבים פיזיולוגיים, בחשיפה במקרה זה שלושה ריכוזים שונים חמצן השראה: היפוקסיה, normoxia ו hyperoxia, הוא תיאר. טכניקה זו מנצלת האדם ריאתי טכניקות מחקר בפיזיולוגיה בסביבה סריקת MRI.

דימות תהודה מגנטית כימות זלוף ריאתי באמצעות מכויל תיוג ספין העורקים

This demonstrates a MR imaging method to measure the spatial distribution of pulmonary blood flow in healthy subjects 
during normoxia (inspired O2, ...

magnetic-resonance-imaging-quantification-pulmonary-perfusion-using

Research

JoVE Journal

Medicine

13.4K Views.  University of California San Diego - UCSD. שיטת הדמיה MR ללמוד את התפלגות זרימת דם ריאתי תחת מגוון רחב של מצבים פיזיולוגיים, בחשיפה במקרה זה שלושה ריכוזים שונים חמצן השראה: היפוקסיה, normoxia ו hyperoxia, הוא תיאר. טכניקה זו מנצלת האדם ריאתי טכניקות מחקר בפיזיולוגיה בסביבה סריקת MRI.

Video: Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking

Purpose: An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that strain is a more sensitive and earlier marker for contractile dysfunction than the frequently used parameter EF. Current technologies for CMR are time consuming and difficult to implement in clinical practice. Feature tracking is a technology that can lead to more automization and robustness of quantitative analysis of medical images with less time consumption than comparable methods.
Methods: An automatic or manual input in a single phase serves as an initialization from which the system starts to track the displacement of individual patterns representing anatomical structures over time. The specialty of this method is that the images do not need to be manipulated in any way beforehand like e.g. tagging of CMR images.
Results: The method is very well suited for tracking muscular tissue and with this allowing quantitative elaboration of myocardium and also blood flow.
Conclusions: This new method offers a robust and time saving procedure to quantify myocardial tissue and blood with displacement, velocity and deformation parameters on regular sequences of CMR imaging. It therefore can be implemented in clinical practice.

An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that strain is a more sensitive and earlier marker for contractile dysfunction than the frequently used parameter EF.

תהודה מגנטית נגזר מסננים הערכה שריר הלב באמצעות מעקב אחר תכונות

Purpose: An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of living systems. Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using qMRI methods. The MRI acquisition protocol begins with localizer images (used to locate the position of the body and tissue of interest within the MRI system), quality control measurements of relevant magnetic field distributions, and structural imaging for general anatomical characterization. The qMRI portion of the protocol includes measurements of the longitudinal and transverse relaxation time constants (T1 and T2, respectively). Also acquired are diffusion-tensor MRI data, in which water diffusivity is measured and used to infer pathological processes such as edema. Quantitative magnetization transfer imaging is used to characterize the relative tissue content of macromolecular and free water protons. Lastly, fat-water MRI methods are used to characterize fibro-adipose tissue replacement of muscle. In addition to describing the data acquisition and analysis procedures, this paper also discusses the potential problems associated with these methods, the analysis and interpretation of the data, MRI safety, and strategies for artifact reduction and protocol optimization.

Neuromuscular diseases often exhibit a temporally varying, spatially heterogeneous, and multi-faceted pathology. The goal of this protocol is to characterize this pathology using non-invasive magnetic resonance imaging methods.

דימות תהודה מגנטית כמותי של מחלת שריר שלד

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

Dynamic changes in tissue water diffusion and glucose metabolism occur during and after hypoxia in cerebral hypoxia-ischemia reflecting a bioenergetics disturbance in affected cells. Diffusion weighted magnetic resonance imaging (MRI) identifies regions that are damaged, potentially irreversibly, by hypoxia-ischemia. Alterations in glucose utilization in the affected tissue may be detectable by positron emission tomography (PET) imaging of 2-deoxy-2-(18F)fluoro-&#7429;-glucose ([18F]FDG) uptake. Due to the rapid and variable nature of injury in this animal model, acquisition of both modes of data must be performed simultaneously in order to meaningfully correlate PET and MRI data. In addition, inter-animal variability in the hypoxic-ischemic injury due to vascular differences limits the ability to analyze multi-modal data and observe changes to a group-wise approach if data is not acquired simultaneously in individual subjects. The method presented here allows one to acquire both diffusion-weighted MRI and [18F]FDG uptake data in the same animal before, during, and after the hypoxic challenge in order to interrogate immediate physiological changes.

The method presented here uses simultaneous positron emission tomography and magnetic resonance imaging. In the cerebral hypoxia-ischemia model, dynamic changes in diffusion and glucose metabolism occur during and after injury. The evolving and irreproducible damage in this model necessitates simultaneous acquisition if meaningful multi-modal imaging data are to be acquired.

ההדמיה PET / MRI בו זמנית בעכבר מוחין היפוקסיה-איסכמיה

Dynamic changes in tissue water diffusion and glucose metabolism occur during and after hypoxia in cerebral hypoxia-ischemia reflecting a bioenergetics ...

Cardiac Magnetic Resonance Imaging at 7 Tesla

CMR at an ultra-high field (magnetic field strength B0&#160;&#8805; 7 Tesla) benefits from the signal-to-noise ratio (SNR) advantage inherent at higher magnetic field strengths and potentially provides improved signal contrast and spatial resolution. While promising results have been achieved, ultra-high field CMR is challenging due to energy deposition constraints and physical phenomena such as transmission field non-uniformities and magnetic field inhomogeneities. In addition, the magneto-hydrodynamic effect renders the synchronization of the data acquisition with the cardiac motion difficult. The challenges are currently addressed by explorations into novel magnetic resonance technology. If all impediments can be overcome, ultra-high field CMR may generate new opportunities for functional CMR, myocardial tissue characterization, microstructure imaging or metabolic imaging. Recognizing this potential, we show that multi-channel radio frequency (RF) coil technology tailored for CMR at 7 Tesla together with higher order B0 shimming and a backup signal for cardiac triggering facilitates high fidelity functional CMR. With the proposed setup, cardiac chamber quantification can be accomplished in examination times similar to those achieved at lower field strengths. To share this experience and to support the dissemination of this expertise, this work describes our setup and protocol tailored for functional CMR at 7 Tesla.

The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.

דימות תהודה מגנטית לב בטסלה 7

CMR at an ultra-high field (magnetic field strength B0&#160;&#8805; 7 Tesla) benefits from the signal-to-noise ratio (SNR) advantage inherent at higher ...

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous studies have shown that abnormal blood flow may be associated with systemic vascular risk. Thus, PC-MRI can facilitate the translation of data obtained from animal models of cardiovascular diseases to pertinent clinical investigations. In this report, we describe the procedure for measuring blood flow in the common carotid artery (CCA) of rats using cine-gated PC-MRI and discuss relevant analysis methods. This procedure can be performed in a live, anesthetized animal and does not require euthanasia after the procedure. The proposed scanning parameters yield repeatable measurements for blood flow, indicating excellent reproducibility of the results. The PC-MRI procedure described in this article can be used for pharmacological testing, pathophysiological assessment, and cerebral hemodynamics evaluation.

The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.

שלב ניגודיות תהודה מגנטית בעורק החולדה נפוצים

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous ...

Quantification of Levator Ani Hiatus Enlargement by Magnetic Resonance Imaging in Males and Females with Pelvic Organ Prolapse

Here we present a protocol to examine the levator ani hiatus in males and females with pelvic organ prolapse, during the Valsalva maneuver and while evacuating acoustic gel, using a horizontally oriented 1.5 T magnetic resonance (MR) scanner. On midsagittal images, the vertical distance of pelvic organs is measured in millimeters relative to the hymen plane (female) and to the lower border of the symphysis pubis (male), preceded by - (above) or + ( below) signs. On axial images, the levator ani area is calculated in square centimeters with a free-hand tracing method from three key images, passing through the midsymphysis (level I), tangential to the lower border of the symphysis (level II), and at the maximal anterior rectal wall bulging (level III). Areas at rest and strained are compared to find evidence of a percentage of increase. The purpose is to provide objective evidence of the maximal extent of pelvic organs descent and hiatus enlargement without the interference of foreign objects or the examiner&#39;s proximity, so as to overcome the limitations of pelvic examination and transperineal sonography (i.e., subjectivity and sex-related limitations [female only]).

Here we present a protocol to standardize the measurement of the levator ani hiatus size&#160;by magnetic resonance imaging. The purpose is to extract biomechanical inferences from image analysis by comparing resting and straining values in patients of both sexes with pelvic prolapse, using consistent anatomical bony landmarks.

כימות של מרים Ani היאטוס הגדלה על ידי תהודה מגנטית של זכרים ונקבות עם צניחת אברי האגן

Here we present a protocol to examine the levator ani hiatus in males and females with pelvic organ prolapse, during the Valsalva maneuver and while ...

Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques

We present the conventional cardiac magnetic resonance (CMR) protocol for evaluating a suspected thrombus and highlight emerging techniques.&#160;The appearance of a mass on certain magnetic resonance (MR) sequences can help differentiate a thrombus from competing diagnoses such as a tumor. T1 and T2 signal characteristics of a thrombus are related to the evolution of hemoglobin properties. A thrombus typically does not enhance following contrast administration, which also helps differentiation from a tumor. We also highlight the emerging role of T1 mapping in the evaluation of a thrombus, which can add another level of support in diagnosis. Prior to any CMR exam, patient screening and interviews are critical to ensure safety and to optimize patient comfort. Effective communication during the exam between the technologist and the patient promotes proper breath holding technique and higher quality images. Volumetric post processing and structured reporting are helpful to ensure that the radiologist answers the ordering services&#39; question and communicates these results effectively. Optimal pre-MR safety evaluation, CMR exam execution, and post exam processing and reporting allow for delivery of high quality radiological service in the evaluation of a suspected cardiac thrombus.

The goal of this article is to describe how cardiac magnetic resonance can be used for the evaluation and diagnosis of a suspected cardiac thrombus. The method presented will describe data acquisition as well as the pre-procedure and post-procedure protocol.

תהודה מגנטית קרדיולוגית להערכת החשוד בתרובוס הלב: טכניקות קונבנציונליות והמתעוררים

We present the conventional cardiac magnetic resonance (CMR) protocol for evaluating a suspected thrombus and highlight emerging techniques.&#160;The ...

Quantitative Mapping of Specific Ventilation in the Human Lung using Proton Magnetic Resonance Imaging and Oxygen as a Contrast Agent

Specific ventilation imaging (SVI) is a functional magnetic resonance imaging technique capable of quantifying specific ventilation &#8213; the ratio of the fresh gas entering a lung region divided by the region&#8217;s end-expiratory volume &#8213; in the human lung, using only inhaled oxygen as a contrast agent. Regional quantification of specific ventilation has the potential to help identify areas of pathologic lung function. Oxygen in solution in tissue shortens the tissue&#8217;s longitudinal relaxation time (T1), and thus a change in tissue oxygenation can be detected as a change in T1-weighted signal with an inversion recovery acquired image. Following an abrupt change between two concentrations of inspired oxygen, the rate at which lung tissue within a voxel equilibrates to a new steady-state reflects the rate at which resident gas is being replaced by inhaled gas. This rate is determined by specific ventilation. To elicit this sudden change in oxygenation, subjects alternately breathe 20-breath blocks of air (21% oxygen) and 100% oxygen while in the MRI scanner. A stepwise change in inspired oxygen fraction is achieved through use of a custom three-dimensional (3D)-printed flow bypass system with a manual switch during a short end-expiratory breath hold. To detect the corresponding change in T1, a global inversion pulse followed by a single shot fast spin echo sequence was used to acquire two-dimensional T1-weighted images in a 1.5&#160;T MRI scanner, using an eight-element torso coil. Both single slice and multi-slice imaging are possible, with slightly different imaging parameters. Quantification of specific ventilation is achieved by correlating the time-course of signal intensity for each lung voxel with a library of simulated responses to the air/oxygen stimulus. SVI estimations of specific ventilation heterogeneity have been validated against multiple breath washout and proved to accurately determine the heterogeneity of the specific ventilation distribution.

Specific ventilation imaging is a functional magnetic resonance imaging technique that allows for quantification of regional specific ventilation in the human lung, using inhaled oxygen as a contrast agent. Here, we present a protocol to collect and analyze specific ventilation imaging data.

מיפוי כמותי של אוורור ספציפי בריאה האנושית באמצעות דימות תהודה מגנטית פרוטון וחמצן כסוכן ניגודיות

Specific ventilation imaging (SVI) is a functional magnetic resonance imaging technique capable of quantifying specific ventilation &#8213; the ratio of ...

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation

Magnetic resonance imaging (MRI) is an important tool for the clinical assessment of cardiovascular morphology and heart function. It is also the recognized standard-of-care for blood flow quantification based on phase contrast MRI. While such measurement of blood flow has been possible in adults for decades, methods to extend this capability to fetal blood flow have only recently been developed.
Fetal blood flow quantification in major vessels is important for monitoring fetal pathologies such as congenital heart disease (CHD) and fetal growth restriction (FGR). CHD causes alterations in the cardiac structure and vasculature that change the course of blood in the fetus. In FGR, the path of blood flow is altered through the dilation of shunts such that the oxygenated blood supply to the brain is increased. Blood flow quantification enables assessment of the severity of the fetal pathology, which in turn allows for suitable&#160;in utero&#160;patient management and planning for postnatal care.
The primary challenges of applying phase contrast MRI to the human fetus include small blood vessel size, high fetal heart rate, potential MRI data corruption due to maternal respiration, unpredictable fetal movements, and lack of conventional cardiac gating methods to synchronize data acquisition. Here, we describe recent technical developments from our lab that have enabled the quantification of fetal blood flow using phase contrast MRI, including advances in accelerated imaging, motion compensation, and cardiac gating.

Here we present a protocol for measuring fetal blood flow rapidly with MRI and retrospectively performing motion correction and cardiac gating.

כימות זרימת דם עוברי אנושי עם הדמיית תהודה מגנטית ופיצוי תנועה

Magnetic resonance imaging (MRI) is an important tool for the clinical assessment of cardiovascular morphology and heart function. It is also the ...

Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla

The overall goal of this article is to demonstrate a state-of-the-art ultrahigh field (UHF) magnetic resonance (MR) protocol of the brain at 7.0 Tesla in multiple sclerosis (MS) patients. MS is a chronic inflammatory, demyelinating, neurodegenerative disease that is characterized by white and gray matter lesions. Detection of spatially and temporally disseminated T2-hyperintense lesions by the use of MRI at 1.5 T and 3 T represents a crucial diagnostic tool in clinical practice to establish accurate diagnosis of MS based on the current version of the 2017 McDonald criteria. However, the differentiation of MS lesions from brain white matter lesions of other origins can sometimes be challenging due to their resembling morphology at lower magnetic field strengths (typically 3 T). Ultrahigh field MR (UHF-MR) benefits from increased signal-to-noise ratio and enhanced spatial resolution, both key to superior imaging for more accurate and definitive diagnoses of subtle lesions. Hence, MRI at 7.0 T has shown encouraging results to overcome the challenges of MS differential diagnosis by providing MS-specific neuroimaging markers (e.g., central vein sign, hypointense rim structures and differentiation of MS grey matter lesions). These markers and others can be identified by other MR contrasts other than T1 and T2 (T2*, phase, diffusion) and substantially improve the differentiation of MS lesions from those occurring in other neuroinflammatory conditions such as neuromyelitis optica and Susac syndrome. In this article, we describe our current technical approach to study cerebral white and grey matter lesions in MS patients at 7.0 T using different MR acquisition methods. The up-to-date protocol includes the preparation of the MR setup including the radio-frequency coils customized for UHF-MR, standardized screening, safety and interview procedures with MS patients, patient positioning in the MR scanner and acquisition of dedicated brain scans tailored for examining MS.

Here, we present a protocol to acquire magnetic resonance (MR) images of multiple sclerosis (MS) patient brains at 7.0 Tesla. The protocol includes preparation of the setup including the radio-frequency coils, standardized interview procedures with MS patients, subject positioning in the MR scanner and MR data acquisition.

הדמיית תהודה מגנטית של טרשת נפוצה ב 7.0 טסלה

The overall goal of this article is to demonstrate a state-of-the-art ultrahigh field (UHF) magnetic resonance (MR) protocol of the brain at 7.0 Tesla in ...