Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic NeuronsAmélie Weiss 1, Peter Sommer 1, Johannes H. Wilbertz 1
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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical modulations.