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London School of Hygiene and Tropical Medicine

4 ARTICLES PUBLISHED IN JoVE

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Medicine

An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Lien Anh Ha Do 1,2, Reuben Tse 1, Jordan Nathanielsz 1, Jeremy Anderson 1, Darren Suryawijaya Ong 1, Keith Chappell 3, Kim Mulholland 1,2,4, Paul V. Licciardi 1,2
1Murdoch Children's Research Institute, 2Department of Paediatrics, University of Melbourne, 3School of Chemistry and Molecular Bioscience, University of Queensland, 4London School of Hygiene and Tropical Medicine

This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types.

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Immunology and Infection

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
Thomas Labadie 1, Quentin Grassin 2, Christophe Batéjat 2, Jean-Claude Manuguerra 2, India Leclercq 2,3
1Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), 3Cellule Pasteur, Université de Paris

Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.

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Immunology and Infection

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Vanessa S. Terra 1, Charles D. Plumptre 2, Emma C. Wall 3, Jeremy S. Brown 2, Brendan W. Wren 1
1Faculty of Infections and Tropical Diseases, Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Division of Medicine, University College London, 3Division of Infection and Immunity, University College London UCL

Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.

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Environment

Visualizing Field Data Collection Procedures of Exposure and Biomarker Assessments for the Household Air Pollution Intervention Network Trial in India
Karthikeyan D. Rajamani 1, Sankar Sambandam 1, Krishnendu Mukhopadhyay 1, Naveen Puttaswamy 1, Gurusamy Thangavel 1, Durairaj Natesan 1, Rengaraj Ramasamy 1, Saritha Sendhil 1, Amudha Natarajan 1, Vigneswari Aravindalochan 1, Ajay Pillarisetti 2, Michael Johnson 3, Joshua Rosenthal *4, Kyle Steenland 5, Ricardo Piedhrahita 3, Jennifer Peel 6, Maggie L. Clark 6, Dana Boyd Barr 5, Sarah Rajkumar 6, Bonnie Young 6, Shirin Jabbarzadeh 7, Ghislaine Rosa 8, Miles Kirby 9, Lindsay J. Underhill 10, Anaite Diaz-Artiga 11, Amy Lovvorn 5, William Checkley 12, Thomas Clasen 5, Kalpana Balakrishnan 1
1Department of Environmental Health Engineering, ICMR Center for Advanced Research on Air Quality, Climate and Health, Faculty of Public Health, Sri Ramachandra Institute of Higher Education and Research (Deemed University), 2Division of Environmental Health Sciences, University of California, Berkeley, 3Berkeley Air Monitoring Group, 4Division of International Epidemiology and Population Studies, National Institutes of Health, 5Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, 6Department of Environmental and Radiological Health Sciences, Colorado State University, 7Department of Biostatistics and Informatics, Rollins School of Public Health, Emory University, 8Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, 9Department of Global Health & Population, Harvard, T.H. Chan School of Public Health, 10Cardiovascular Division, Washington University School of Medicine, Washington University, 11Centro de Estudios en Salud, Universidad del Valle de Guatemala, 12Division of Pulmonary and Critical Care, School of Medicine, Johns Hopkins University

We detail the consistent, high-quality procedures used throughout air and biological sampling processes at Indian field sites during a large randomized controlled trial. Insights gathered from the oversight of applications of innovative technologies, adapted for exposure assessment in rural regions, enable better field data collection practices with more reliable outcomes.

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