Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting AssayJulian Rapp *1,2, Jan Ness *1,3, Paula Liang 1, Martin J. Hug 4, Hansjürgen Agostini 1, Günther Schlunck 1, Clemens Lange 5, Felicitas Bucher 1
1Eye Center, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 2Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 3Institute of Pharmaceutical Sciences, Faculty of Chemistry and Pharmacy, University of Freiburg, 4Pharmacy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Ophtha-Lab, Department of Ophthalmology, St. Franziskus Hospital Muenster
This study presents the two-dimensional (2D) scratch wound migration assay and the three-dimensional (3D) spheroid sprouting assay, along with their respective downstream analysis methods, including RNA extraction and immunocytochemistry, as suitable assays to study angiogenesis in vitro.
