Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

1.2K Views

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09:16 min

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May 31st, 2024

DOI :

10.3791/66954-v

May 31st, 2024

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Julian Rapp*¹^,², Jan Ness*¹^,³, Paula Liang¹, Martin J. Hug⁴, Hansjürgen Agostini¹, Günther Schlunck¹, Clemens Lange⁵, Felicitas Bucher¹

¹Eye Center, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ²Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ³Institute of Pharmaceutical Sciences, Faculty of Chemistry and Pharmacy, University of Freiburg, ⁴Pharmacy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ⁵Ophtha-Lab, Department of Ophthalmology, St. Franziskus Hospital Muenster

Summary

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Scratch Wound Migration Assay

Spheroid Sprouting Assay

Molecular Mechanisms

In Vitro Assays

Endothelial Cells

Human Umbilical Vein Endothelial Cells HUVECs

Neovascular Disease

Therapeutic Options

Collagen Matrix

Live Cell Imaging

Assay Comparison

Translational Research

Chapters in this video

0:00

Introduction

1:30

Scratch Wound Migration Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

3:13

Fixing and Blocking 2D Human Umbilical Vein Endothelial Cell (HUVEC) Cultures for Immunohistochemical Analysis

4:49

Spheroid Sprouting Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

7:41

Immunocytochemistry of Human Umbilical Vein Endothelial Cell (HUVEC) Spheroids in a 3D Collagen Matrix for Investigating Angiogenesis In Vitro

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