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University of Science and Technology Beijing

8 ARTICLES PUBLISHED IN JoVE

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Engineering

Measurement of Coherence Decay in GaMnAs Using Femtosecond Four-wave Mixing
Daniel Webber 1, Tristan de Boer 1, Murat Yildirim 1, Sam March 1, Reuble Mathew 1, Angela Gamouras 1, Xinyu Liu 2, Margaret Dobrowolska 2, Jacek Furdyna 2, Kimberley Hall 1
1Department of Physics and Atmospheric Science, Dalhousie University, 2Department of Physics, University of Notre Dame

The technique of femtosecond four-wave mixing is described, including spectrally-resolved and time-resolved configurations. We illustrate the utility of this technique for the investigation of crucial physical properties in the III-V diluted magnetic semiconductors, afforded by its nonlinearity and high temporal resolution.

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JoVE Journal

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants
Junlian Wang 1, Meiying Xie 1, Xinyu Liu 1, Shengming Xu 2
1School of Civil and Resource Engineering, University of Science and Technology Beijing, 2Institute of Nuclear and New Energy Technology, Tsinghua University

A protocol for the synthesis of high purity nonsymmetric dialkylphosphinic acid extractants is presented, taking (2,3-dimethylbutyl)(2,4,4'-trimethylpentyl)phosphinic acid as an example.

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JoVE Journal

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
Jiang Li 1, Jia Man 2, Zhongnan Li 2, Haosheng Chen 2
1School of Mechanical Engineering, University of Science and Technology Beijing, 2State Key Laboratory of Tribology, Tsinghua University

A phase-inversion co-flow device is demonstrated to generate monodisperse high-viscosity droplets above 1 Pas, which is difficult to realize in droplet microfluidics.

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Engineering

Quantitative Analysis by Thermogravimetry-Mass Spectrum Analysis for Reactions with Evolved Gases
Rongbin Li 1, Qian Huang 2, Kai Wei 2, Hongde Xia 2
1School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, 2Institute of Engineering Thermophysics, Chinese Academy of Sciences

Precise determination of the evolved gases' flow rate is key to study the details of reactions. We provide a novel quantitative analysis method of equivalent characteristic spectrum analysis for thermogravimetry-mass spectrum analysis by establishing the calibration system of the characteristic spectrum and relative sensitivity, for obtaining the flow rate.

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Environment

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
Zhuo Li 1,2, Fangting Ai 3, Jing Zhang 4, Zhenyang Yu 1,2, Daqiang Yin 1,2
1College of Environmental Sciences and Engineering, Tongji University, 2Shanghai Institute of Pollution Control and Ecological Security, 3Jiaxing Tongji Institute of Environment, 4College of Ecological Technique and Engineering, Shanghai Institute of Technology

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

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Engineering

Measurement of Chladni Mode Shapes with an Optical Lever Method
Rong Feng *1, Yan Luo *1, Yixue Dong 1, Mengke Ma 2, Yuqi Wang 2, Jing Zhang 3, Wenjiang Ma 3, Donghuan Liu 4
1School of Materials Science and Engineering, University of Science and Technology Beijing, 2School of Civil and Resource Engineering, University of Science and Technology Beijing, 3Basic Experimental Center for Natural Science, University of Science and Technology Beijing, 4School of Mathematics and Physics, University of Science and Technology Beijing

A simple method of measuring the Chladni mode shape on an elastic plate by the principle of an optical lever is proposed.

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Cancer Research

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Jing Zhang 1, Midory Thorikay 1, Gerard van der Zon 1, Maarten van Dinther 1, Peter ten Dijke 1
1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

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Cancer Research

High-Resolution Ultrasonography for the Analysis of Orthotopic ATC Tumors in a Genetically Engineered Mouse Model
Yushuang He 1, Yan Luo 1, Zhihui Li 2, Han Luo 2, Huayun Yan 3, Yingfang Ma 3, Xinyue Zhou 2
1Department of Ultrasound, West China Hospital, Sichuan University, 2Department of Thyroid and Parathyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, 3State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center

The present protocol describes high-frequency ultrasonography for visualizing the entire mouse thyroid gland and monitoring the growth of anaplastic thyroid carcinoma.

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