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Hannover medical school

13 ARTICLES PUBLISHED IN JoVE

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Medicine

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Dorothée Sturm 1,2, Lorella Marselli 3, Florian Ehehalt 1,2, Daniela Richter 1, Marius Distler 2, Stephan Kersting 1,2, Robert Grützmann 2, Krister Bokvist 4, Philippe Froguel 5, Robin Liechti 6, Anne Jörns 7, Paolo Meda 8, Gustavo Bruno Baretton 9, Hans-Detlev Saeger 2, Anke M. Schulte 10, Piero Marchetti 3, Michele Solimena 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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Developmental Biology

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Claudia Davenport 1, Ulf Diekmann 1, Ortwin Naujok 1
1Institute of Clinical Biochemistry, Hannover Medical School

Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

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Medicine

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach
Nodir Madrahimov 1, Ruslan Natanov 1, Erin C. Boyle 1, Tobias Goecke 1, Ann-Kathrin Knöfel 1, Valentyna Irkha 1, Anna Solovieva 2, Klaus Höffler 1, Ulrich Maus 3, Christian Kühn 1, Issam Ismail 1, Gregor Warnecke 1, Malakh-Lal Shrestha 1, Serghei Cebotari *1, Axel Haverich *1
1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, 3Department of Pneumology, Hannover Medical School

This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.

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Characterization of the Interaction of Primary Cells from the Rat Inner Ear with Polymer Films As Coatings for Cochlear Implant Electrode Surface
Gudrun Brandes 1, Christoph Hadler 2, Athanasia Warnecke 3, Jasmin Bohlmann 3, Wiebke Dempwolf 2, Henning Menzel 2, Thomas Lenarz 3, Kirsten Wissel 3
1Institute of Neuroanatomy and Cell Biology, Hannover Medical School, 2Institute for Technical Chemistry, University of Technology Braunschweig, 3Department of Otorhinolaryngology, Hannover Medical School

Here, we present an immunocytochemical and electron microscopical protocol that enables qualitative and quantitative characterization of the interaction of the primary spiral ganglion neurons and other cell types within ultrathin polymer films.

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Immunology and Infection

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
Vanessa Neuhaus *1, Olga Danov *1, Sebastian Konzok 1, Helena Obernolte 1, Susann Dehmel 1, Peter Braubach 2, Danny Jonigk 2, Hans-Gerd Fieguth 3, Patrick Zardo 4, Gregor Warnecke 4, Christian Martin 5, Armin Braun 1,6, Katherina Sewald 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School

In view of the 3Rs principle, respiratory models as alternatives to animal studies are evolving. Especially for risk assessment of respiratory substances, there is a lack of appropriate assays. Here, we describe the use of human precision-cut lung slices for the assessment of airborne substances.

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Medicine

Veno-Venous Extracorporeal Membrane Oxygenation in a Mouse
Nodir Madrahimov 1, Abdurasul Khalikov 1, Erin C. Boyle 1, Ruslan Natanov 1, Ann-Kathrin Knoefel 1, Thierry Siemeni 1, Klaus Hoeffler 1, Axel Haverich 1, Ulrich Maus *2, Christian Kuehn *1
1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Pneumology, Hannover Medical School

Here we present a protocol describing the technique of veno-venous extracorporeal membrane oxygenation (ECMO) in a non-intubated, spontaneously breathing mouse. This murine model of ECMO can be effectively implemented in experimental studies of acute and end-stage lung diseases.

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Immunology and Infection

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
Clara A. Weigle 1, Thorsten Lieke 1, Florian W. R. Vondran 1, Kai Timrott 1, Jürgen Klempnauer 1, Oliver Beetz 1
1Regenerative Medicine and Experimental Surgery, Department of General, Visceral and Transplant Surgery, Hannover Medical School

We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

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Medicine

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model
Decheng Yin *1,2, Jian Fu *1,3, Ida Allabauer 1, Oliver Witzke 4, Song Rong 5, André Hoerning 1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, 5Department of Nephrology, Hannover Medical School

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

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Immunology and Infection

A Modified Surgical Technique for Kidney Transplantation in Mice
Decheng Yin *1,2, Jian Fu *1,3, Rongjun Chen 4, Nelli Shushakova 5, Ida Allabauer 1, Xin-Yi Wei 1, Mario Schiffer 6, Diana Dudziak 7, Song Rong *5, André Hoerning *1
1Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 2Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, 3The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, 4Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, 5Department of Nephrology, Hannover Medical School, 6Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, 7Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

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Neuroscience

Topographical EEG Recordings of Visual Evoked Potentials in Mice using Multichannel Thin-film Electrodes
Rüdiger Land 1, Sarah Christine Sentis 1,2, Andrej Kral 1
1Institute for Audioneurotechnology, Department of Experimental Otology, Hannover Medical School, 2Institute for Endocrinology and Diabetes, Center of Brain, Behavior and Metabolism, University of Lübeck

The present protocol describes a simple procedure to acquire and analyze the topography of epicranial visual evoked potentials with 32-multichannel thin-film electrodes in the mouse.

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Medicine

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
Oliver Beetz *1, Felix Oldhafer *1, Clara A. Weigle 1, Sebastian Cammann 1, Daphne DeTemple 1, Lion Sieg 2, Hendrik Eismann 2, Thomas Palmaers 2, Florian W. R. Vondran 1
1Department of General, Visceral and Transplant Surgery, Hannover Medical School, 2Department of Anaesthesiology and Intensive Care Medicine, Hannover Medical School

In this protocol, a model of porcine orthotopic liver transplantation after static cold storage of donor organs for 20 h without the use of a veno-venous bypass during engraftment is described. The approach uses a simplified surgical technique with minimization of the anhepatic phase and sophisticated volume and vasopressor management.

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Medicine

Phase-Resolved Functional Lung MRI for Pulmonary Ventilation and Perfusion (V/Q) Assessment
Andreas Voskrebenzev 1,2, Filip Klimeš 1,2, Frank Wacker 1,2, Jens Vogel-Claussen 1,2
1Institute for Diagnostic and Interventional Radiology, Hannover Medical School, 2Member of the German Center for Lung Research, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)

Here, we describe the implementation of phase-resolved functional lung MRI as a contrast-agent-free proton MR technique for the assessment of pulmonary ventilation and perfusion dynamics. Validated and applicable across different field strengths and age groups, it could enhance clinical decision-making in the future by aiding in disease quantification and therapy monitoring.

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Medicine

Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging
Filip Klimeš 1,2, Andreas Voskrebenzev 1,2, Frank Wacker 1,2, Jens Vogel-Claussen 1,2
1Institute of Diagnostic and Interventional Radiology, Hannover Medical School, 2Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Centre for Lung Research (DZL)

Three-dimensional (3D) phase-resolved functional lung (PREFUL) is a functional magnetic resonance imaging (MRI) technique that allows for quantification of regional ventilation of the whole human lung volume, using tidal breathing and contrast-agent free acquisition for 8 min. Here, we present an MR protocol to collect and analyze 3D PREFUL imaging data.

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