A Modified Surgical Technique for Kidney Transplantation in Mice

5.5K Views

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10:38 min

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July 22nd, 2022

DOI :

10.3791/63434-v

July 22nd, 2022

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Decheng Yin*¹^,², Jian Fu*¹^,³, Rongjun Chen⁴, Nelli Shushakova⁵, Ida Allabauer¹, Xin-Yi Wei¹, Mario Schiffer⁶, Diana Dudziak⁷, Song Rong*⁵, André Hoerning*¹

¹Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, ⁵Department of Nephrology, Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg

Summary

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Kidney Transplantation

Mice

Surgical Technique

Vessel Anastomosis

Ureteral Implantation

Aorta Ligation

Renal Artery

Donor Left Renal Vein

Anesthesia

Isoflurane

Intraperitoneal Injection

Chapters in this video

0:05

Introduction

1:09

Preparatory Steps

1:43

Donor Operation Procedure

3:26

Recipient Operation Procedure

7:29

Ureteral Implantation

9:19

Representative Results

10:12

Conclusion

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