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University of Konstanz

7 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe
Ana F. Silbering 1, Rati Bell 1, C. Giovanni Galizia 2, Richard Benton 1
1Center for Integrative Genomics, University of Lausanne, 2Department of Biology, University of Konstanz

We describe an established technique to measure and analyze odor-evoked calcium responses in the antennal lobe of living Drosophila melanogaster.

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Neuroscience

Appetitive Associative Olfactory Learning in Drosophila Larvae
Anthi A. Apostolopoulou 1, Annekathrin Widmann 1, Astrid Rohwedder 1,2, Johanna E. Pfitzenmaier 1, Andreas S. Thum 1,2
1Department of Biology, University of Konstanz, 2Department of Biology, University of Fribourg

Drosophila larvae are able to associate odor stimuli with gustatory reward. Here we describe a simple behavioral paradigm that allows the analysis of appetitive associative olfactory learning.

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Developmental Biology

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
Katherine W. Rogers 1, Alexander Bläßle 2, Alexander F. Schier 1, Patrick Müller 2
1Department of Molecular and Cellular Biology, Harvard University, 2Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society

Protein levels in cells and tissues are often tightly regulated by the balance of protein production and clearance. Using Fluorescence Decay After Photoconversion (FDAP), the clearance kinetics of proteins can be experimentally measured in vivo.

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Developmental Biology

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
Vaibhav Shinde *1, Stefanie Klima *2, Perumal Srinivasan Sureshkumar 1, Kesavan Meganathan 1, Smita Jagtap 1, Eugen Rempel 3, Jörg Rahnenführer 3, Jan Georg Hengstler 4, Tanja Waldmann 2, Jürgen Hescheler 1, Marcel Leist *2, Agapios Sachinidis *1
1Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, 2Department of Biology, University of Konstanz, 3Department of Statistics, Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund

The protocols describe two in vitro developmental toxicity test systems (UKK and UKN1) based on human embryonic stem cells and transcriptome studies. The test systems predict human developmental toxicity hazard, and may contribute to reduce animal studies, costs and the time required for chemical safety testing.

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Neuroscience

Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera
Eirik Søvik *1,2, Jenny A. Plath *3,4, Jean-Marc Devaud 5, Andrew B. Barron 3
1Department of Science and Mathematics, Volda University College, 2Department of Biology, Washington University in St. Louis, 3Department of Biological Sciences, Macquarie University, 4Department of Biology, University of Konstanz, 5Research Center on Animal Cognition, CNRS, Universite de Toulouse

This manuscript describes several protocols for administering pharmacological agents to honey bees, including simple noninvasive methods for free-flying bees, as well as more invasive variants that allow precise localized treatment of restrained bees.

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Developmental Biology

A Simple and Effective Transplantation Device for Zebrafish Embryos
Gary H. Soh 1, Anna C. Kögler 1, Patrick Müller 1,2
1Systems Biology of Development Group, Friedrich Miescher Laboratory of the Max Planck Society, 2University of Konstanz

Embryological manipulations such as extirpation and transplantation of cells are important tools to study early development. This protocol describes a simple and effective transplantation device to perform these manipulations in zebrafish embryos.

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Medicine

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
Hang You 1,2, Xin Mao 2, Chenyang Wang 2, Gang Huang 3, Marcus Groettrup 4,5, Jun Li 2
1School of Medicine, Chongqing University, 2Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, 4Division of Immunology, Department of Biology, University of Konstanz, 5Biotechnology Institute Thurgau, University of Konstanz

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

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