Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
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17:28 min
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June 17th, 2015
DOI :
June 17th, 2015
•Vaibhav Shinde*1, Stefanie Klima*2, Perumal Srinivasan Sureshkumar1, Kesavan Meganathan1, Smita Jagtap1, Eugen Rempel3, Jörg Rahnenführer3, Jan Georg Hengstler4, Tanja Waldmann2, Jürgen Hescheler1, Marcel Leist*2, Agapios Sachinidis*1
1Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, 2Department of Biology, University of Konstanz, 3Department of Statistics, Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund
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Chapters in this video
0:05
Title
2:02
H9 Cell Culture on Basement Membrane Matrix Coated Plates
4:40
Embryoid Bodies Formation
6:17
Collection of Embryoid Bodies and Treatment
7:55
RNA Isolation
9:31
Splitting and Maintenance of H9 Cells
10:55
Differentiation of hESC towards Neuroectodermal Progenitor Cells (NEP)
15:06
Results: Methyl Mercury Exposure Causes Gene Expression Changes in Embryoid Bodies
16:40
Conclusion
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