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University of Nebraska - Kearney

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
April Maschmann 1, Cody Masters 1, Melissa Davison 1, Joshua Lallman 1, Drew Thompson 1, Kristy L. Kounovsky-Shafer 1
1Department of Chemistry, University of Nebraska - Kearney

Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.

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