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University of Innsbruck

6 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
Eva Wagner *1,2,3, Sören Brandenburg *1,2, Tobias Kohl 1,2, Stephan E. Lehnart 1,2,3,4
1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine

In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.

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Environment

The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants
Michael Wisniewski 1, Gilbert Neuner 2, Lawrence V. Gusta 3
1U.S Department of Agriculture, Agricultural Research Service (USDA-ARS), Kearneysville, WV, 2Institute of Botany, University of Innsbruck, 3Department of Plant Science, University of Saskatechewan

Here we present a protocol that allows one to visualize sites of ice formation and avenues of ice propagation in plants utilizing high resolution infrared thermography (HRIT).

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Biochemistry

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Alexander K. H. Weiss 1,2, Max Holzknecht 1,2, Elia Cappuccio 1,2, Ilaria Dorigatti 1, Karin Kreidl 1, Andreas Naschberger 3, Bernhard Rupp 3, Hubert Gstach 4, Pidder Jansen-Dürr 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

Expression and purification of fumarylacetoacetate hydrolase domain-containing proteins is described with examples (expression in E. coli, FPLC). Purified proteins are used for crystallization and antibody production and employed for enzyme assays. Selected photometric assays are presented to display the multi-functionality of FAHD1 as oxaloacetate decarboxylase and acylpyruvate hydrolase.

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Environment

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Klemens Weisleitner 1,2, Lars Hunger 3, Christoph Kohstall 4, Albert Frisch 5, Michael C. Storrie-Lombardi 6, Birgit Sattler 1,2
1Institute of Ecology, University of Innsbruck, 2Austrian Polar Research Institute, University of Vienna, 3BrainLinks-BrainTools, Bernstein Center Freiburg, 4Atom Science, Kasevich Lab, Stanford University, 5Institute of Experimental Physics, University of Innsbruck, 6Department of Physics, Extraterrestrial Vehicle Instruments Laboratory, Harvey Mudd College

Carbon fluxes in the cryosphere are hardly assessed yet but are crucial regarding climate change. Here we show a novel prototype device that captures the phototrophic potential in supraglacial environments based on laser-induced fluorescence emission (L.I.F.E.) technology offering high spectral and spatial resolution data under in situ conditions.

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Biology

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Alexander Lichius 1, Susanne Zeilinger 1
1Department of Microbiology, University of Innsbruck

Vital fluorescent dyes are essential tools for live-cell imaging analyses in modern fungal cell biology. This paper details the application of established and lesser-known fluorescent dyes for tracking plasma membrane dynamics, endo-/exocytosis and cell wall morphogenesis in filamentous fungi.

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Biochemistry

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
Andreas Andric 1, Eva Wagner 1, Anne Heberle 1, Max Holzknecht 1, Alexander K. H. Weiss 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.

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