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JoVE Journal

Bioengineering

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Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

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10:30 min

October 15th, 2014

October 15th, 2014

20,345 Views

0:05

Title

1:46

Isolation of Atrial and Ventricular Myocytes from the Mouse Heart

4:09

Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes

5:41

Analysis of the TATS Membrane Network and its Components

8:23

Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes

9:26

Conclusion

In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.

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