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Braunschweig University of Technology

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Kenric J. Hoegler 1, Martin Distel 2, Reinhard W. Köster 3, John H. Horne 1
1Department of Biology, Pace University, 2Cellular and Molecular Medicine, University of California, San Diego, 3Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

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Developmental Biology

Culture and Transfection of Zebrafish Primary Cells
Giulio Russo 1,2, Franziska Lehne 1, Sol M. Pose Méndez 1, Stefan Dübel 2, Reinhard W. Köster 1, Wiebke A. Sassen 1
1Division of Cellular and Molecular Neurobiology, Zoological Institute, Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig University of Technology

We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.

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Neuroscience

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
Paul Schramm 1, Florian Hetsch 2, Jochen C. Meier 3, Reinhard W. Köster 1
1Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute, Technische Universität Braunschweig

Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention.

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