Preparation of Primary Cells from Zebrafish Embryos
1:54
Preparation of Reusable Poly-L-lysine-coated Glass Bottom Dishes
3:26
Preparation and Plating of Primary Cells
6:02
Transfection of Primary Cells with Plasmid DNA
6:57
Results: Live Cell Imaging of Zebrafish Primary Cells
8:25
Conclusion
Transcript
This method provides the zebrafish community a tool for the the analysis of intercellular dynamics with a resolution unattainable in vivo. And also it allows to compensate for the lack of zebrafish cell lines. The main advantage of this technique
Sign in or start your free trial to access this content
We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.