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University of Waikato

2 ARTICLES PUBLISHED IN JoVE

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Genetics

Measuring Microbial Mutation Rates with the Fluctuation Assay
Rok Krašovec 1, Huw Richards 2,3, Guillaume Gomez 3, Danna R. Gifford 1, Adrien Mazoyer 4, Christopher G. Knight 3
1School of Biological Sciences, The University of Manchester, 2School of Science, University of Waikato, 3School of Natural Sciences, The University of Manchester, 4Department of Mathematics, Université du Québec à Montréal

Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

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Biochemistry

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Ronja Stelzer 1, Elizabeth Rzoska-Smith 1, Sigurd Gundesø 2, Ulli Rothweiler 2, Adele Williamson 1
1University of Waikato, 2ArcticZymes Technologies ASA

Here, a protocol for assaying nucleic acid metabolizing enzymes is presented, using examples of ligase, nuclease, and polymerase enzymes. The assay utilizes fluorescently labeled and unlabeled oligonucleotides that can be combined to form duplexes mimicking RNA and/or DNA damages or pathway intermediates, allowing for the characterization of enzyme behavior.

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