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Measuring Microbial Mutation Rates with the Fluctuation Assay (Video) | JoVE

DOI :

10.3791/60406-v

7:44 min

November 28th, 2019

November 28th, 2019

21,532 Views

1School of Biological Sciences, The University of Manchester, 2School of Science, University of Waikato, 3School of Natural Sciences, The University of Manchester, 4Department of Mathematics, Université du Québec à Montréal

Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

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Measuring Microbial Mutation Rates

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