Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem CellsAshley Ulm 1, Christopher N. Mayhew 2, Jason Debley 3, Gurjit K. Khurana Hershey 4, Hong Ji 1,4
1Pyrosequencing Core, Cincinnati Children's Hospital, 2Division of Developmental Biology, Cincinnati Children's Hospital, 3Division of Pulmonary Medicine, Seattle Children's Hospital, 4Division of Asthma Research, Cincinnati Children's Hospital
This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).