ST. JOSEPH'S HOSPITAL AND MEDICAL CENTER

2 ARTICLES PUBLISHED IN JoVE

Biology

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Landon J. Inge¹, Aaron J. Fowler¹, Ross M. Bremner¹

¹Center for Thoracic Disease and Transplantation, Heart and Lung Institute, St. Joseph's Hospital and Medical Center

There is a discernible need for improved information on molecular drivers of Barrett’s Esophagus. Immunofluorescent staining is a useful technique for understanding the effects of cell signaling on cell morphology. We present a simple, effective protocol for the use of immunofluorescent staining to assess therapeutic treatment in Barrett’s Esophagus cells.

Immunology and Infection

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Deepak K. Nayak¹, Oscar Mendez¹, Sara Bowen², Thalachallour Mohanakumar¹

¹Norton Thoracic Institute, St. Joseph's Hospital and Medical Center, ²Flow Cytometry Core, St. Joseph's Hospital and Medical Center

This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.