Juntendo University School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Shun Ishiyama^1,2,3,4, Casey Kissel⁵, Xin Guo¹, Alexis Howard⁶, Harumi Saeki⁷, Tomoaki Ito⁸, Polina Sysa-Shah⁹, Hajime Orita¹⁰, Kazuhiro Sakamoto⁴, Kathleen Gabrielson^1,2

¹Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, ²Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, ³Department of Surgery, Johns Hopkins University School of Medicine, ⁴Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, ⁵Program for Comparative Medicine, Gene Therapy Program, Perelman School of Medicine, University of Pennsylvania, ⁶Tuskegee College of Veterinary Medicine, ⁷Department of Human Pathology, Juntendo University Faculty of Medicine, ⁸Department of Surgery, Juntendo University Shizuoka Hospital, Juntendo University School of Medicine, ⁹Department of Urology, The Johns Hopkins School of Medicine, ¹⁰Department of Gastroenterology and Minimally Invasive Surgery, Juntendo University Faculty of Medicine

Here, a syngeneic orthotopic implantation followed by an amputation procedure of the osteosarcoma with spontaneous pulmonary metastasis that can be used for preclinical investigation of metastasis biology and development of novel therapeutics is described.

Cancer Research

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Rachel Vistein¹, Briana Winer², Stephanie Myers^1,3, Juliane Liberto⁴, Shun Ishiyama^1,5,6, Xin Guo¹, Harumi Saeki⁷, Tian-Li Wang^4,8, Ie-Ming Shih^2,4,8, Kathleen Gabrielson^1,4,8

¹Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, ²Department of Obstetrics and Gynecology, Johns Hopkins University School of Medicine, ³Texas Tech University School of Veterinary Medicine, ⁴Department of Pathology, Johns Hopkins University School of Medicine, ⁵Department of Surgery, Johns Hopkins University School of Medicine, ⁶Department of Coloproctological Surgery, Juntendo University School of Medicine, ⁷Department of Human Pathology, Juntendo University School of Medicine, ⁸Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University

This protocol describes a method for monitoring the progression of morphological changes over time in the uterus in an inducible mouse model of endometrial cancer using ultrasound imaging with correlation to gross and histological changes.