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Shiga University of Medical Science

1 ARTICLES PUBLISHED IN JoVE

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Biology

An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
Mariko Omatsu-Kanbe 1, Ryo Fukunaga 1, Xinya Mi 1, Hiroshi Matsuura 1
1Department of Physiology, Shiga University of Medical Science

We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

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