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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

DOI :

10.3791/61866-v

8:33 min

May 19th, 2021

May 19th, 2021

7,937 Views

1Department of Physiology, Shiga University of Medical Science

We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

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Antegrade Perfusion Method

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