JoVE Logo

Sign In

Korea Advanced Institute of Science and Technology (KAIST)

9 ARTICLES PUBLISHED IN JoVE

image

Medicine

Breathing-controlled Electrical Stimulation (BreEStim) for Management of Neuropathic Pain and Spasticity
Sheng Li 1,2,3
1Department of Physical Medicine and Rehabilitation, University of Texas Health Science Center at Houston , 2UTHealth Motor Recovery Laboratory, TIRR Memorial Hermann Hospital, 3The Institute of Rehabilitation and Research (TIRR), TIRR Memorial Hermann Hospital

The purpose is to present a new method, breathing-control electrical stimulation (BreEStim) for management of neuropathic pain and spasticity.

image

Bioengineering

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
Jaejung Son 1, Chae Yun Bae 1, Je-Kyun Park 1
1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)

We describe the fabrication of micropatterned hydrogel sheets using a simple process, which can be assembled and manipulated in a freestanding form. Using these modular hydrogel sheets, a simple macro-scaled 3D cell culture system can be generated with a controlled cellular microenvironment.

image

Bioengineering

TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application
Keumyeon Kim 1, Haeshin Lee 1,2, Seonki Hong 2,3
1The Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), 2Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 3Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School

We describe the simplest protocol to prepare biodegradable medical glue that has an effective hemostatic ability. TAPE is a water-immiscible supramolecular aggregate prepared by mixing of tannic acid, a ubiquitous compound found in plants, and poly(ethylene) glycol, yielding a 2.5 times greater water-resistant adhesion compared with commercial fibrin glue.

image

Chemistry

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
Sura Kim 1, Jayoung Ku 1,2, Jaemin Park 1, Raisa Kharbash 1,2, Sheng Li 1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through immunoprecipitation.

image

Neuroscience

Three-Dimensional Shape Modeling and Analysis of Brain Structures
Jaeil Kim 1, Maria del Carmen Valdés Hernández 2, Jinah Park 3
1School of Computer Science and Engineering, Kyungpook National University, 2Centre for Clinical Brain Sciences, University of Edinburgh, 3School of Computing and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

We introduce a semi-automatic protocol for shape analysis on brain structures, including image segmentation using open software, and further group-wise shape analysis using an automated modeling package. Here, we demonstrate each step of the 3D shape analysis protocol with hippocampal segmentation from brain MR images.

image

Biochemistry

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Sujin Kim 1, Minjeong Kang 1, Yoosik Kim 1
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

We present experimental approaches for studying RNA-interactors of double-stranded RNA binding protein kinase RNA-activated (PKR) during the mammalian cell cycle using HeLa cells. This method utilizes formaldehyde to crosslink RNA-PKR complexes and immunoprecipitation to enrich PKR-bound RNAs. These RNAs can be further analyzed through high-throughput sequencing or qRT-PCR.

image

Biology

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Alicia Masters 1,2, Minjeong Kang 1,3,4, Morgan McCaw 1,3, Jacob D. Zobrist 1,3,5, William Gordon-Kamm 2, Todd Jones 2, Kan Wang 1,3
1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University

Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.

image

Neuroscience

Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
Han-Sol Lee 1,2, Jin-Hee Han 1,2
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 2KAIST Institute for BioCentury (KIB), Korea Advanced Institute of Science and Technology (KAIST)

In vivo microendoscopic calcium imaging is an invaluable tool that enables real-time monitoring of neuronal activities in freely behaving animals. However, applying this technique to the amygdala has been difficult. This protocol aims to provide a useful guideline for successfully targeting amygdala cells with a miniaturized microscope in mice.

image

Developmental Biology

Applications of RNA Interference in American Cockroach
Liang Li *1, Andi Jing *1, Minxin Xie 1, Sheng Li 1,2, Chonghua Ren 1,2
1Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, 2Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University

The present protocol describes step-by-step guidelines for the RNAi operation techniques in P. americana.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved