JoVE Logo

Sign In

Winship Cancer Institute of Emory University

2 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
David R. Myers *1,2,3,4, Yumiko Sakurai *1,2,3,4, Reginald Tran 1,2,3,4, Byungwook Ahn 1,2,3,4, Elaissa Trybus Hardy 1,2,3,4, Robert Mannino 1,2,3,4, Ashley Kita 1,2,3,4, Michelle Tsai 1,2,3,4, Wilbur A. Lam 1,2,3,4
1Department of Pediatrics, Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , 4Winship Cancer Institute of Emory University

A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.

image

Medicine

Segmentation and Linear Measurement for Body Composition Analysis using Slice-O-Matic and Horos
Sean Steele 1, Fangyi Lin 1, Thien-Linh Le 1, Alexandra Medline 1, Michelle Higgins 1, Alex Sandberg 1, Sean Evans 1, Gordon Hong 2, Milton A. Williams 3, Mehmet A. Bilen 4, Sarah Psutka 5, Kenneth Ogan 1, Viraj A. Master 1
1Department of Urology, Emory University School of Medicine, 2Northeast Ohio Medical University, 3Department of Urology, University of Alabama at Birmingham, 4Department of Medical Oncology, Winship Cancer Institute of Emory University, 5Department of Urology, University of Washington

Segmentation and linear measurements quantify skeletal muscle mass and adipose tissues using Computed Tomography and/or Magnetic Resonance Imaging images. Here, we outline the use of Slice-O-Matic software and Horos image viewer for rapid and accurate analysis of body composition. These methods can provide important information for prognosis and risk stratification.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved