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Children's National Medical Center

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Labeling and Imaging Cells in the Zebrafish Hindbrain
Pradeepa Jayachandran 1, Elim Hong 2, Rachel Brewster 1
1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center

Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.

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Biology

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
Anjali A. Sarkar 1, Irene E. Zohn 1
1Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center

The cranial mesenchyme undergoes dramatic morphogenic movements that likely provides a driving force for elevation of the neural folds1,2. Here we describe a simple ex vivo explant assay to characterize the cellular behaviors of the cranial mesenchyme during neurulation. This assay has numerous applications including being amenable to pharmacological manipulations and live imaging analyses.

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Biology

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair
Aurelia Defour *1, S. C. Sreetama *1, Jyoti K. Jaiswal 1,2
1Center for Genetic Medicine Research, Children's National Medical Center, 2Department of Integrative Systems Biology, George Washington University

The process of healing injured cells involves trafficking of specific proteins and subcellular compartments to the site of cell membrane injury. This protocol describes assays to monitor these processes.

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Behavior

Behavioral and Locomotor Measurements Using an Open Field Activity Monitoring System for Skeletal Muscle Diseases
Kathleen S. Tatem 1, James L. Quinn 1, Aditi Phadke 1, Qing Yu 1, Heather Gordish-Dressman 1,2, Kanneboyina Nagaraju 1,2
1Center for Genetic Medicine Research, Children's National Medical Center, 2Department of Integrative Systems Biology, George Washington University School of Medicine and Health Sciences

Open field activity levels are used to assess locomotive and behavioral activity levels. This protocol provides a well-designed, standardized protocol to use in preclinical trials for neuromuscular disorders.

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Bioengineering

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
Jennifer M. Vojtech 1,2, Juliana Cano-Mejia 1,2, Matthieu F. Dumont 1, Raymond W. Sze 1,3, Rohan Fernandes 1,3,4
1The Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Medical Center, 2Fischell Department of Bioengineering, University of Maryland, 3Department of Radiology, George Washington University, 4Department of Pediatrics, George Washington University

This protocol describes the synthesis of biofunctionalized Prussian blue nanoparticles and their use as multimodal, molecular imaging agents. The nanoparticles have a core-shell design where gadolinium or manganese ions within the nanoparticle core generate MRI contrast. The biofunctional shell contains fluorophores for fluorescence imaging and targeting ligands for molecular targeting.

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