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Sheba Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells
Asher Castiel 1, Leonid Visochek 2,3, Leonid Mittelman 4, Yael Zilberstein 4, Francoise Dantzer 5, Shai Izraeli 1,6, Malka Cohen-Armon 2,3
1Cancer Research Center, Sheba Medical Center, 2The Neufeld Cardiac Research Institute, Tel-Aviv University, 3Department of Physiology and Pharmacology, Tel-Aviv University, 4Imaging Unit, Sackler Faculty of Medicine, Tel-Aviv University, 5Biotechnology and Cell Signaling, Ecole Superieure de Biotechnologie Strasbourg, 6Department of Human Molecular Genetics and Biochemistry, Tel-Aviv University

The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.

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Biology

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Ayelet Di Segni 1, Tzipi Braun 1, Marina BenShoshan 1, Sarit Farage Barhom 1, Efrat Glick Saar 1, Karen Cesarkas 1, James E. Squires 2, Nathan Keller 1,3, Yael Haberman 1,4
1Sheba Medical Center, 2Children's Hospital of Pittsburgh of UPMC, 3Ariel University, 4Cincinnati Children's Hospital Medical Center

This manuscript describes a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from fecal sample collection through data analyses. This protocol enables analysis of large numbers of samples with rigorous standards and several controls.

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JoVE Core

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Lael Werner 1,2, Chen Dor 3,4, Naomi Salamon 1, Meital Nagar 3,4, Dror S. Shouval 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

The current protocol describes a method for DNA isolation from blood samples and intestinal biopsies, generation of TCRβ and IGH PCR libraries for next-generation sequencing, performance of a NGS run and basic data analysis.

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