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University Hospital Heidelberg

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Ann-Kristin Mueller 1, Jochen Behrends 2, Jannike Blank 2, Ulrich E. Schaible 2, Bianca E. Schneider 2
1Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, 2Priority Area Infections, Research Center Borstel

Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of infection.

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Medicine

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
Angelika Hoffmann 1,2, Xavier Helluy 2,3, Manuel Fischer 2, Ann-Kristin Mueller 4,5, Sabine Heiland 2, Mirko Pham 1,6, Martin Bendszus 1, Johannes Pfeil 4,5,7
1Department of Neuroradiology, Heidelberg University Hospital, 2Division of Experimental Radiology, Department of Neuroradiology, Heidelberg University Hospital, 3NeuroImaging Centre Research, Department of Neuroscience, Ruhr-University Bochum, 4Centre for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, 5German Centre for Infection Research (DZIF), 6Department of Neuroradiology, University of Würzburg, 7Center for Childhood and Adolescent Medicine, General Pediatrics, Heidelberg University Hospital

We describe a mouse model of experimental cerebral malaria and show how inflammatory and microvascular pathology can be tracked in vivo using magnetic resonance imaging.

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Medicine

Subcutaneous Trigeminal Nerve Field Stimulation for Refractory Facial Pain
Martin Jakobs 1, Sigrid Schuh-Hofer 2, Andreas Unterberg 1, Rezvan Ahmadi 1
1Department of Neurosurgery, University Hospital Heidelberg, 2Center for Biomedicine and Medical Technology, University Hospital Mannheim

Treating chronic or neuropathic facial pain can be challenging when medical or standard treatment fails. Subcutaneous nerve field stimulation is the least invasive form of neuromodulation and is used for chronic back pain. We applied this technology to treat chronic and neuropathic trigeminal facial pain.

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Developmental Biology

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
Soah Lee *1,2,3, Huaxiao Yang *1,2,3, Caressa Chen *1,2,3, Sneha Venkatraman 1,2,3, Adrija Darsha 1,2,3, Sean M. Wu 1,2,3, Joseph C. Wu 1,2,3, Timon Seeger 1,2,3,4,5
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim

This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.

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Cancer Research

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
Ashwin Narayanan 1, Enrique Blanco-Carmona 2, Engin Demirdizen 1, Xueyuan Sun 1,3, Christel Herold-Mende 4, Matthias Schlesner 2, Sevin Turcan 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

Intra-tumoral heterogeneity is an inherent feature of tumors, including gliomas. We developed a simple and efficient protocol that utilizes a combination of buffers and gradient centrifugation to isolate single nuclei from fresh frozen glioma tissues for single nucleus RNA and ATAC sequencing studies.

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