Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

7.4K Views

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08:59 min

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April 3rd, 2020

DOI :

10.3791/60888-v

April 3rd, 2020

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Soah Lee*¹^,²^,³, Huaxiao Yang*¹^,²^,³, Caressa Chen*¹^,²^,³, Sneha Venkatraman¹^,²^,³, Adrija Darsha¹^,²^,³, Sean M. Wu¹^,²^,³, Joseph C. Wu¹^,²^,³, Timon Seeger¹^,²^,³^,⁴^,⁵

¹Stanford Cardiovascular Institute, Stanford University School of Medicine, ²Department of Medicine, Division of Cardiovascular Medicine, Stanford University, ³Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, ⁴Department of Medicine III, University Hospital Heidelberg, ⁵German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim

Summary

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Lithography free

Single cell Micropatterning

Laser cut Stencils

Microfabrication

Mechanotransduction

High throughput Drug Screening

Disease Modeling

Hydrogel Solution

Photoinitiator Solution

Basement Membrane Matrix

UV Cross linkable Polyacrylamide

Sterilization Techniques

Biological Safety Hood

Chapters in this video

0:04

Introduction

0:55

Solution Preparation

1:48

Hydrogel Fabrication

3:43

Protein Conjugation

6:24

Results: Micropatterning of Cells

7:52

Conclusion

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