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National Institute of Advanced Industrial Science and Technology (AIST)

7 ARTICLES PUBLISHED IN JoVE

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Environment

Protocol for Measuring the Thermal Properties of a Supercooled Synthetic Sand-water-gas-methane Hydrate Sample
Michihiro Muraoka 1, Naoko Susuki 1, Hiroko Yamaguchi 2, Tomoya Tsuji 3, Yoshitaka Yamamoto 1
1Research Institute of Energy Frontier, National Institute of Advanced Industrial Science and Technology (AIST), 2College of Industrial Technology, Nihon University, 3SHIZEN ikohza, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia

We present a protocol for measuring the thermal properties of synthetic hydrate-bearing sediment samples comprising sand, water, methane, and methane hydrate.

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Engineering

Micro/Nano-scale Strain Distribution Measurement from Sampling Moiré Fringes
Qinghua Wang 1, Shien Ri 1, Hiroshi Tsuda 1
1Research Institute for Measurement and Analytical Instrumentation, National Institute of Advanced Industrial Science and Technology (AIST)

A sampling moiré technique featuring 2-pixel and multi-pixel sampling methods for high-accuracy strain distribution measurements at the micro/nano-scale is presented here.

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Chemistry

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
Toshiaki Koga 1, Yoshitaro Sakata 1, Nao Terasaki 1
1Advanced Manufacturing Research Institute, Department of Electronics and Manufacturing, National Institute of Advanced Industrial Science and Technology (AIST)

Here, accumulation of cuprous ions in a copper sulfate plating solution in a model experiment and an analysis based on quantitative measurements are described. This experiment reproduces the accumulation process of cuprous ions in the plating bath.

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Immunology and Infection

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
Masamune Morita 1, Yuri Ota 1,2, Kaoru Katoh 1, Naohiro Noda 1,2
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2Department of Life Science and Medical Bioscience, Waseda University

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.

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Genetics

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
Yukie Akutsu 1, Motomichi Doi 1, Koji Furukawa 1, Yuko Takagi 1
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Here, we describe a protocol to introduce a gene knockout into the extracellular amastigote of Trypanosoma cruzi, using the CRISPR/Cas9 system. The growth phenotype can be followed up either by cell counting of axenic amastigote culture or by proliferation of intracellular amastigotes after host cell invasion.

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Developmental Biology

Electroporation-mediated RNA Interference Method in Odonata
Genta Okude 1,2, Takema Fukatsu 1,2,3, Ryo Futahashi 2
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 2Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences, University of Tsukuba

We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).

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Education

Mechanoluminescent Visualization of Crack Propagation for Joint Evaluation
Nao Terasaki 1, Yuki Fujio 1
1Sensing System Research Center (SSRC), National Institute of Advanced Industrial Science and Technology (AIST)

In this study, a protocol is presented that describes the use of mechanoluminescent (ML) visualization for monitoring crack propagation and mechanical behavior during adhesive joint evaluation testing.

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