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Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

DOI :

10.3791/59555-v

April 30th, 2019

April 30th, 2019

6,683 Views

1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2Department of Life Science and Medical Bioscience, Waseda University

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.

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Keywords Bacterial Cell Culture

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