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Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

4 ARTICLES PUBLISHED IN JoVE

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Biology

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
Deborah Mattinzoli 1, Piergiorgio Messa 2, Alessandro Corbelli 1, Masami Ikehata 1, Anna Mondini 1, Cristina Zennaro 3, Silvia Armelloni 1, Min Li 1, Laura Giardino 1, Maria Pia Rastaldi 1
1Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models. 

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Neuroscience

Brain Morphology of Cannabis Users With or Without Psychosis: A Pilot MRI Study
Giuseppe Delvecchio *1, Lucio Oldani *2, Gian Mario Mandolini 2, Alessandro Pigoni 3, Valentina Ciappolino 2, Giandomenico Schiena 2, Matteo Lazzaretti 2, Elisabetta Caletti 2, Viviana Barbieri 1, Claudia Cinnante 4, Fabio Triulzi 1,4, Paolo Brambilla 1,2
1Department of Pathophysiology and Transplantation, University of Milan, 2Department of Neurosciences and Mental Health, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3MoMiLab Research Unit, IMT School for Advanced Studies Lucca, 4Neuroradiology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

This is a 3T magnetic resonance imaging study aiming to investigate grey matter volume differences between cannabis-induced psychosis patients and non-psychotic chronic cannabis users.

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Medicine

Transthoracic Echocardiography to Assess Post-Resuscitation Left Ventricular Dysfunction After Acute Myocardial Infarction and Cardiac Arrest in Pigs
Daria De Giorgio 1, Davide Olivari 1, Francesca Fumagalli 1, Lidia Staszewsky 1, Giuseppe Ristagno 2,3
1Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2University of Milan, 3Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

Transthoracic echocardiography is the first-line diagnostic test for post-resuscitation left ventricular dysfunction and structural changes in a pig model of cardiac arrest.

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Biology

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Min Li *1, Hongkun Qin *1, Yunfei Long 1, Meiqin Cheng 1, Ling Li 1, Aijun Huang 2, Nian Wang 3, Shuo Duan 1
1China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, Gannan Normal University, 2Department of Biological Sciences, Gannan Normal University, 3Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida - Institute of Food and Agricultural Sciences

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

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