In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice CulturesEissa Alfadil 1, Frank Bradke 1, Sebastian Dupraz 1
1Laboratory of Axon Growth and Regeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.