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JoVE Journal

Neuroscience

This content is Free Access.

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

3.0K Views

10:45 min

October 14th, 2021

October 14th, 2021

3,032 Views

0:05

Introduction

1:11

Embryo Extraction

1:44

Ex Utero and In Utero Electroporation

3:35

Brain Extraction and Embedding in Agarose

4:39

Organotypic Slice Culture

5:43

Image Acquisition and Data Analysis

8:29

Results: In Situ Axon Growth and Growth Cone Dynamics

9:56

Conclusion

Transcript

This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

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