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Instituto de Salud Carlos III (ISCIII)

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
Alfonso Rubio-Navarro 1, Melania Guerrero-Hue 1, Beatriz Martín-Fernandez 2, Isabel Cortegano 3, Elena Olivares-Alvaro 2, Natalia de las Heras 2, Mario Alía 3, Belén de Andrés 3, María Luisa Gaspar 3, Jesús Egido 1, Juan Antonio Moreno 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information obtained in the experimental model.

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Biology

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
Silvia Murillo-Cuesta *1,2,3, Néstor Vallecillo *1, Rafael Cediel 1,2,4, Adelaida M. Celaya 1,2, Luis Lassaletta 3,5, Isabel Varela-Nieto 1,2,3, Julio Contreras 1,2,4
1Instituto de Investigaciones Biomédicas (IIBm) Alberto Sols CSIC-UAM, 2Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), 3Instituto de Investigación Sanitaria La Paz (IdiPAZ), 4Facultad de Veterinaria, Universidad Complutense de Madrid, 5Departmento de Otorrino laringología, Hospital Universitario La Paz

Two microsurgery approaches for local drug delivery to the inner ear are described here and compared in terms of impact on hearing parameters, cochlear cytoarchitecture and expression of inflammatory markers.

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Medicine

Mobile Device-assisted Dietary Ecological Momentary Assessments for the Evaluation of the Adherence to the Mediterranean Diet in a Continuous Manner
Anna Boronat 1, Joana Clivillé-Pérez 2, Natalia Soldevila-Domenech 1,2, Laura Forcano 1,3, Nieves Pizarro 1,2, Montserrat Fitó 3,4, Helmut Schröder 4,5, Karine Fauria 6, Rafael de la Torre 1,2,3
1Integrative Pharmacology and Systems Neurosciences Research Group, Neurosciences Research Program, Hospital del Mar Medical Research Institute (IMIM), 2Department of Experimental and Health Sciences, University Pompeu Fabra, 3CIBER de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, 4Cardiovascular Risk and Nutrition Research Group, Hospital del Mar Medical Research Institute (IMIM), 5CIBER de Epidemiología y Salud Pública (CIBERESP), Instituto de Salud Carlos III (ISCIII), 6Barcelonaβeta Brain Research Center (BBRC), Pasqual Maragall Foundation

Presented here is a study protocol aimed at monitoring continuous adherence to the Mediterranean diet (MedDiet) by means of ecological momentary assessments. The method evaluates the intake of key food groups of the MedDiet and calculates an index of adherence.

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