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Shiv Nadar University

2 ARTICLES PUBLISHED IN JoVE

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Environment

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Deepa Sethi 1, Richa Priyadarshini 1
1Department of Life Sciences, School of Natural Sciences, Shiv Nadar University

We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

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Genetics

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
Shalini Soni 1, Shubhra Agarwal 1, Rakesh Aggarwal 2, Naga Suresh Veerapu 1
1Virology Section, Department of Life Sciences, Shiv Nadar University, 2Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.

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