1.2K Views
•
15:49 min
•
June 9th, 2022
DOI :
June 9th, 2022
•Chapters in this video
0:04
Introduction
1:01
Genome‐Wide Substitution Mutagenesis of JFH1 Using Error‐Prone PCR
2:46
Estimation of the Proportion of Mutations in ep‐PCR Products (Mutant Libraries)
6:08
Viral RNA Transfection of the Huh7.5 Cell Line
7:57
Quantification of Virus Titers
11:59
Drug‐Resistant Viral Variant Selection
13:33
Results: Integration of ep‐PCR and Virus Reverse Genetics to Generate HCV Mutants
15:17
Conclusion
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