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Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

DOI :

10.3791/63685-v

June 9th, 2022

June 9th, 2022

1,001 Views

1Virology Section, Department of Life Sciences, Shiv Nadar University, 2Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.

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