Yeungnam University

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Yun-Ui Bae¹, Hoon-Ki Sung^2,3, Jae-Ryong Kim¹

¹Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, ²Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, ³Department of Laboratory Medicine and Pathobiology, University of Toronto

This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.

Immunology and Infection

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Jun-O Jin^1,2, Soyeong Jang³, Hyehyun Kim⁴, Junghwan Oh⁴, Sungbo Shim⁵, Minseok Kwak^3,4, Peter C.W. Lee⁶

¹Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, ²Department of Medical Biotechnology, Yeungnam University, ³Department of Chemistry, Pukyong National University, ⁴Marine-integrated Bionics Research Center, Pukyong National University, ⁵Department of Biochemistry, Chungbuk National University, ⁶Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial.

Biology

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Yanqing Jiang^*1, Joanna Lan-Hing Yeung^*1, Ju Hee Lee^1,2, James An¹, Patrick E. Steadman³, Jae-Ryong Kim⁴, Hoon-Ki Sung^1,2,5

¹Translational Medicine Program, The Hospital for Sick Children, ²Department of Laboratory Medicine and Pathobiology, University of Toronto, ³Neurosciences & Mental Health Program, The Hospital for Sick Children, ⁴Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine, Yeungnam University, ⁵Banting and Best Diabetes Centre, University of Toronto

The focus of the present study is to demonstrate the whole-mount immunostaining and visualization technique as an ideal method for 3D imaging of adipose tissue architecture and cellular component.