Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

10.2K Views

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09:32 min

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January 8th, 2017

DOI :

10.3791/55035-v

January 8th, 2017

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Yun-Ui Bae¹, Hoon-Ki Sung²^,³, Jae-Ryong Kim¹

¹Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, ²Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, ³Department of Laboratory Medicine and Pathobiology, University of Toronto

Summary

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Keywords Mouse Embryonic Stem Cells

Senescence associated Beta galactosidase

Chapters in this video

0:05

Title

0:49

Mouse Embryonic Stem Cell (mESC) Culture

2:06

Serum- and Feeder-free Conditioned Medium Collection

3:17

Senescence-associated (SA) Beta Galactosidase Assay

4:46

Cell Cycle and qRT-PCR Analyses

7:19

Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function

8:29

Conclusion

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