Tokoha University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Hideya Kawasaki¹, Isao Kosugi¹, Makiko Sakao-Suzuki², Shiori Meguro¹, Yoshihiro Tsutsui³, Toshihide Iwashita¹

¹Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, ²First Department of Medicine, Hamamatsu University School of Medicine, ³Faculty of Health Science, Tokoha University

Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.