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Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

DOI :

10.3791/54164-v

July 24th, 2016

July 24th, 2016

18,584 Views

1Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, 2First Department of Medicine, Hamamatsu University School of Medicine, 3Faculty of Health Science, Tokoha University

Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.

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