Doshisha Women's College

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Takayuki Ueno^1,2, Kiyoshi Kawasaki², Takeo Kubo¹

¹Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, ²Faculty of Pharmaceutical Sciences, Doshisha Women's College

Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.