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Tecnologico de Monterrey

4 ARTICLES PUBLISHED IN JoVE

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Engineering

Fabrication of 3D Carbon Microelectromechanical Systems (C-MEMS)
Bidhan Pramanick 1, Sergio O. Martinez-Chapa 1, Marc Madou 1,2, Hyundoo Hwang 1,3
1School of Engineering and Sciences, Tecnologico de Monterrey, 2Department of Mechanical and Aerospace Engineering, University of California, 3BBB Inc

Long and hollow glassy carbon microfibers were fabricated based on the pyrolysis of a natural product, human hair. The two fabrication steps of carbon microelectromechanical and carbon nanoelectromechanical systems, or C-MEMS and C-NEMS, are: (i) photolithography of a carbon-rich polymer precursor and (ii) pyrolysis of the patterned polymer precursor.

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Neuroscience

Three-Dimensional Motor Nerve Organoid Generation
Tatsuya Osaki 1,2, Siu Yu A. Chow 1,2, Yui Nakanishi 1,2, Joel Hernández 1,3, Jiro Kawada 4, Teruo Fujii 1, Yoshiho Ikeuchi 1,2
1Institute of Industrial Science, The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 3Faculty of Science and Engineering, Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.

This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.

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Neuroscience

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
Lena Sophie Koch 1, David Choy Buentello 2,3, Kerensa Broersen 1
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

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JoVE Core

Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy
Perla Pérez-Treviño 1, Bianca Nieblas 1,2, Selma Romina López-Vaquera 1, Noemí García 1,2,3
1Experimental Medicine and Advanced Therapies, The Institute for Obesity Research, Tecnologico de Monterrey, 2Escuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, 3Preclinical Research Unit, Tecnologico de Monterrey

Here, we present a protocol to analyze changes in mitochondrial density and longitudinal distribution by live-skeletal muscle imaging using confocal microscopy for mitochondrial network scanning.

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