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German Federal Institute for Risk Assessment (BfR)

8 ARTICLES PUBLISHED IN JoVE

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Biology

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research
Christopher Weidner 1, Matthias Steinfath 1, Elisa Wistorf 1, Michael Oelgeschläger 1, Marlon R. Schneider 1, Gilbert Schönfelder 1,2
1Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin

We provide a standardized protocol for the use of gene set enrichment analysis of transcriptomic data to identify an ideal mouse model for translational research.
This protocol can be used with DNA microarray and RNA sequencing data and can further be extended to other omics data if data are available.

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Behavior

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia
Katharina Hohlbaum 1,2, Bettina Bert 2,3, Silke Dietze 2, Rupert Palme 4, Heidrun Fink 2, Christa Thöne-Reineke 1
1Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, 2Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, 3German Federal Institute for Risk Assessment (BfR), 4Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine

We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.

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JoVE Core

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
Fabian L. Kriegel 1,2, Ralf Köhler 2, Jannike Bayat-Sarmadi 2, Simon Bayerl 3, Anja E. Hauser 2,3, Raluca Niesner 2, Andreas Luch *1, Zoltan Cseresnyes *4
1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, a Leibniz Institute, 3Charité Universitätsmedizin Berlin, 4Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute

Here, we provide a workflow that allows the identification of healthy and pathological cells based on their 3-dimensional shape. We describe the process of using 2D projection outlines based on the 3D surfaces to train a Self-Organizing Map that will provide objective clustering of the investigated cell populations.

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Chemistry

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products
Ines Schreiver 1, Christoph Hutzler 1, Andreas Luch 1
1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

This method for two-step pyrolysis online coupled to gas chromatography with mass spectrometric detection and data evaluation protocol can be used for multi-component analysis of tattoo inks and discrimination of counterfeit products.

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Chemistry

Versatile Dual-Inlet Sample Introduction System for Multi-Mode Single Particle Inductively Coupled Plasma Mass Spectrometry Analysis and Validation
Daniel Rosenkranz 1, Fabian L. Kriegel 1, Emmanouil Mavrakis 2, Spiros A. Pergantis 2, Philipp Reichardt 1, Jutta Tentschert 1, Norbert Jakubowski 4, Peter Laux 1, Ulrich Panne 3, Andreas Luch 1
1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Environmental Chemical Processes Laboratory, Department of Chemistry, University of Crete, 3Federal Institute for Materials Research and Testing (BAM), 4SPETEC GmbH

Here we provide a protocol for the use of a dual-inlet system for single particle inductively coupled mass spectrometry which allows for a standard independent nanoparticle characterization.

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Chemistry

Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
Francesca Bennet 1, Anja Müller 1, Jörg Radnik 1, Yves Hachenberger 2, Harald Jungnickel 2, Peter Laux 2, Andreas Luch 2, Jutta Tentschert 2
1Division of Surface Analysis and Interfacial Chemistry, Federal Institute for Material Research and Testing (BAM), 2Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

A number of different procedures for preparing nanoparticles for surface analysis are presented (drop casting, spin coating, deposition from powders, and cryofixation). We discuss the challenges, opportunities, and possible applications of each method, particularly regarding the changes in the surface properties caused by the different preparation methods.

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Engineering

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
Ana C. Quevedo 1, Emily Guggenheim 1, Sophie M. Briffa 1, Jessica Adams 2,3, Stephen Lofts 2, Minjeong Kwak 4, Tae Geol Lee 4, Colin Johnston 5, Stephan Wagner 6, Timothy R. Holbrook 6, Yves U. Hachenberger 7, Jutta Tentschert 7, Nicholas Davidson 1, Eugenia Valsami-Jones 1
1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2UK Centre for Ecology and Hydrology, 3Natural England, 4Center for Nanosafety Metrology, Korea Research Institute of Standards and Science (KRISS), 5Department of Materials, University of Oxford, 6Department of Analytical Chemistry, Helmholtz-Centre for Environmental Research, 7Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

This study presents the benchmarking results for an interlaboratory comparison (ILC) designed to test the standard operating procedure (SOP) developed for gold (Au) colloid dispersions characterized by ultraviolet-visible Spectroscopy (UV-Vis), amongst six partners from the H2020 ACEnano project for sample preparation, measurement, and analysis of the results.

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Engineering

3D Printing - Evaluating Particle Emissions of a 3D Printing Pen
Heike Sigloch 1, Frank S. Bierkandt 1, Ajay V. Singh 1, Ashish K. Gadicherla 2, Peter Laux 1, Andreas Luch 1
1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Department of Biological Safety, German Federal Institute for Risk Assessment (BfR)

This protocol presents a method to analyze the emission of 3D printing pens. Particle concentration and particle size distribution of the released particle is measured. Released particles are further analyzed with transmission electron microscopy (TEM). Metal content in filaments is quantified by inductively coupled plasma mass spectrometry (ICP-MS).

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