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National Jewish Health

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Charmion Cruickshank-Quinn 1, Kevin D. Quinn 1, Roger Powell 1, Yanhui Yang 1, Michael Armstrong 1, Spencer Mahaffey 2, Richard Reisdorph 1, Nichole Reisdorph 1
1Department of Immunology, National Jewish Health, 2Department of Pharmacology, School of Medicine, University of Colorado Denver

The reliability of results in metabolomics experiments depends on the effectiveness and reproducibility of the sample preparation. Described is a rigorous and in-depth method that enables extraction of metabolites from biological fluids with the option of subsequently analyzing up to thousands of compounds, or just the compound classes of interest.

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Immunology and Infection

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Mia J. Smith 1,2, Thomas A. Packard 1, Shannon K. O'Neill 1, Rochelle M. Hinman 1, Marynette Rihanek 3, Peter A. Gottlieb 3, John C. Cambier 1,4
1Department of Immunology and Microbiology, University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, 4Department of Biomedical Research, National Jewish Health

A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.

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Neuroscience

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
Stacey M. Thomas 1, Joseph Bednarek 2, William J. Janssen 1, Patrick S. Hume 1
1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine, National Jewish Health, 2Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital Colorado

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

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