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Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

DOI :

10.3791/62215-v

February 2nd, 2021

February 2nd, 2021

5,297 Views

1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine, National Jewish Health, 2Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital Colorado

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

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