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Azusa Pacific University

4 ARTICLES PUBLISHED IN JoVE

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Medicine

The Use of Thermal Infra-Red Imaging to Detect Delayed Onset Muscle Soreness
Hani H. Al-Nakhli 1, Jerrold S. Petrofsky 1,2, Michael S. Laymon 2, Lee S. Berk 1
1Loma Linda University, 2Azusa Pacific University

The purpose of this investigation was to assess whether using an infra-red thermal camera is a valid tool for detecting and quantifying the muscle soreness after exercising.

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Behavior

Quantifying Social Motivation in Mice Using Operant Conditioning
Loren Martin 1, Erica Iceberg 1
1Department of Graduate Psychology, Azusa Pacific University

This article describes a new protocol for the quantitative measurement of social motivation in mice using operant conditioning for a social reward. The protocol is useful for the measurement of social motivation in mouse models of autism and other disorders of social behavior.

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Chemistry

Solid-phase Synthesis of [4.4] Spirocyclic Oximes
Cody R. Drisko 1, Silas A. Griffin 1, Kevin S. Huang 1
1Department of Biology and Chemistry, Azusa Pacific University

Here we present a protocol to demonstrate an efficient method for the synthesis of spirocyclic heterocycles. The five-step process utilizes solid-phase synthesis and regenerating Michael linker strategies. Generally difficult to synthesize, we present a customizable method for the synthesis of spirocyclic molecules otherwise inaccessible to other modern approaches.

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Biology

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Amelia N. Gray 1, Breeana M. Ramirez 1, Selom K. Mawugbe 1, Jordan F. Mar 1, Yun-Lan C. Wong 1, Kevin S. Huang 1
1Department of Biology and Chemistry, Azusa Pacific University

Here, we describe a bioassay using 3-(4′,5′-dimethylthiazol-2′-yl)-2,5- diphenyltetrazolium bromide (MTT) to test previously synthesized spirocyclic oximes.

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