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Korea Research Institute of Standards and Science (KRISS)

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
Jina Lee *1,2, Joo-Eun Lee *3, Seil Kim 1,4,5, Dukjin Kang 1, Hee Min Yoo 1
1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST)

Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.

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Engineering

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
Ana C. Quevedo 1, Emily Guggenheim 1, Sophie M. Briffa 1, Jessica Adams 2,3, Stephen Lofts 2, Minjeong Kwak 4, Tae Geol Lee 4, Colin Johnston 5, Stephan Wagner 6, Timothy R. Holbrook 6, Yves U. Hachenberger 7, Jutta Tentschert 7, Nicholas Davidson 1, Eugenia Valsami-Jones 1
1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2UK Centre for Ecology and Hydrology, 3Natural England, 4Center for Nanosafety Metrology, Korea Research Institute of Standards and Science (KRISS), 5Department of Materials, University of Oxford, 6Department of Analytical Chemistry, Helmholtz-Centre for Environmental Research, 7Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

This study presents the benchmarking results for an interlaboratory comparison (ILC) designed to test the standard operating procedure (SOP) developed for gold (Au) colloid dispersions characterized by ultraviolet-visible Spectroscopy (UV-Vis), amongst six partners from the H2020 ACEnano project for sample preparation, measurement, and analysis of the results.

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