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The Affiliated Zhangjiagang Hospital of Soochow University

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
Paul Lu 1,2, Lori Graham 2, Yaozhi Wang 2, Di Wu 2, Mark Tuszynski 1,2
1Veterans Administration Medical Center, San Diego, 2Department of Neurosciences, University of California, San Diego

Fibrin matrices containing growth factors were used to retain grafted neural stem cells into sites of complete spinal cord transection. Grafted cells completely filled the lesion cavity and differentiated into multiple neural cell types, including neurons that extended axons into host spinal cord over long distances.

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JoVE Journal

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite
Li Duan *1,2, Yongli Yin *3, Jia Wang *1, Qi Kang 1,2, Di Wu 1, Huan Jiang 1, Pu Zhang 1, Liang Hu 1
1National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, 2School of Engineering Sciences, University of Chinese Academy of Sciences, 3China Astronaut Research and Training Center

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.

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JoVE Journal

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Di Wu 1, Grzegorz Piszczek 1
1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

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Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones
Yincheng Wang 1, Ke Wang 1, Yuqi Huang 1, Di Wu 2, Jian Wu 3, Jibo He 1
1Department of Psychology, School of Social Sciences, Tsinghua University, 2Department of Computer Science, Beijing Normal University, 3Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

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Biochemistry

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Di Wu 1, Mengfan He 1, Dawei Li 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.

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